During 2019, 24 students worked with me on research and 15 co-authored publications with me: Emma Bush, Ben Mancini, Ashley Hare, Alisha Ungkuldee, Ali Kadouh, Noor Abdallah, Maggie Leff, Matt Cederman, Megan Monforton, Ally Bauer, Danielle Hohman, Oren Lerner, Scott Nolan, Grace Kuza, Zakai Zaman, Maria Green, Jad Mourad, Megan Pastrick, Cameron Carpenter, Michael Reiterman, Katie Bederka, Jacob Wolfe, Joe Kelly and Robert Davison (just too many individuals photos, used lab photo instead).

Noor Abdallah, Ali Kadouh, Ben Mancini, Maria Green, Alex Blundin, Samantha Thacker, Hannah Zawisa and Davin Hami -helped out with CF ion flux, cell culture, and working on publications (at new location, 2140 BPS).

Caleigh Griffin, Andrew Van Alst, Emily Nemeth, Noor Abdallah, Davin Hami, Hannah Zawisa and Allison Vlk -helped out with CF pH, ion flux and cell culture work (and move to new location, 2140 BPS).

Caleigh Griffin, Andrew Van Alst, Andrea Hess, Katrina Price, Samantha Thacker and Alex Tawa -helped out with BioCore data analysis at the CF & STEM Learning lab.

Raj Singh, Samantha Tauscher, Janaan Meyers, Chelsey Klein, Caleigh Griffin & Andrew Van Alst -helped out with the CF lab and teaching.

Candace Igert (now Candace Marquette) graduated with a Masters degree in May 2014

MSU CF Interns 2014

Greg Ribble, Raj Singh, Kathryn Kesler, Ahmad Tahawi, Chelsey Klein, Caleigh Griffin & Andrew Van Alst -helped out with PCR of CFTR, teaching and software content development

Candace Igert

MSU CF Interns 2012-2013

Hillary Albert, Lauren Lendzion, Anthony Dimovski, Nicole Patel, Paul Singh, Nick Fernandez, Lauren Kustasz, Leah Brynaert, Chuck Ternes, Eric Kontowicz, Nicole Rando, Elizabeth DeCesare, Eli Guttman, Jake Aubry and Aaron Rivkin
In 2012-2013 A new graduate student, Candace Igert, joined the CF research lab and several teams of undergraduates worked on correctors. Four teams of three students each used sterile cell culture, ion flux and the cytosensor to collect data regarding "correctors" that help mutant 508CFTR get to the apical membrane of epithelial cells. Hillary Albert, Lauren Lendzion, Anthony Dimovski studied several Dihydrophryridines (DHPs) from Dr. Mazzei's CF research group in Genova Italy; Nicole Patel, Paul Singh and Nick Fernandez studied the drug VX-809 from Vertex Pharmacueticals; Lauren Kustacz, Leah Brynaert and Chuck Ternes evaluated Thapsigargin, and Eric Kontowiscz, Jake Aubry and Aaron Rivkin tested the phosphodiesterase inhibitor (PDE) Sildenafil. Nicole Rando, Elizabeth DeCesare and Eli Guttman also worked in lab during the summer on a variety of cell culture and other projects.

Ben Marengo, Aaron Rivkin, Jake Aubry, Bo Parsons, and Lauren Kustacz
In Spring 2012 Ben, Jake and Aaron continued to work as a team to mine learning data from ten years of student exams and work on research regarding the intervention called the Verbal Final, to master sterile cell culture. Two new students joined the lab, Bo Parsons and Lauren Kustasz, who helped with data collection on the BRAID project.

For two weeks in July 2012 a local high school senior, Neha Buch, and an MSU student, Kevin Wheelock helped out in the lab too.

Lindsey Foos, Ben Marengo, Aaron Rivkin and Jake Aubry
In Summer & Fall 2011 Lindsey, Ben, Jake and Aaron worked as a team in a very focused effort to mine learning data from ten years of student exams, to master sterile cell culture and use the cytosensor to collect data regarding "correctors" that help mutant 508CFTR get to the apical membrane of epithelial cells.

Katie Oleski and Rupal Patel
In Summer 2010 the CF lab worked in Paris, France most the summer and then Katie and Rupal brought some samples back that were isolated and tested by PCR for two weeks in August.

David Maison, Vincent Cracolici, Mitchell Wood, Olivia Shull and Jayme Olsen
In Summer 2009 David, Vincent, Mitch, Olivia and Jayme worked as a team in a very focused effort to create a PCR-based diagnostic test for a collection of different mutations that are known to cause cystic fibrosis.

PSL Graduate Student

Michael Haenisch
In May 2009 Mike won the Meites Award from the Department of Physiology for his research, successfully defended his thesis and completed his Master's thesis manuscript. In summer 2009 he helped prepare his research project to be carried on by other researchers in the lab before heading off to Minnesota for a new job. He worked as a Biological Scientist in the Ranum Lab at the University of Minnesota and University of Florida, currently he is working in the Biotech industry in Seattle, Washington.

Online Citations (Papers and Abstracts)
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Brian Wlosinski, Aaron Lewandowski, Katie Carpenter and Khaled Hammoud
In Spring 2008 Brian, Aaron, Katie and Khaled worked with graduate student Mike Haenisch in his research on how the bacteria [pseudomonas and burkholderia] or their secretions inflamming the CF lung might alter CFTR channel behavior or lead to loss of CFTR channels at the cell surface.

Jamie Geist, Lauren Gamble and Pratima Nayak
In Spring 2007 Jamie Geist continued her study of "BRAIDed learning" and Pratima Nayak continued her research. Lauren Gamble joined the CF lab in May & June to help out with some CF research.

Angela Wright 2006-2007 and Michael Haenisch 2006-2009
Two new graduate students joined the MSU CF lab in June of 2006 and as graduate students in the Department of Physiology.

MSU CF Interns Fall 2006

Jamie Geist, Luke Kane, Devin Murphy and Pratima Nayak
In Fall 2006 Devin Murphy joined the lab to study "AP-PCR techniques" while Jamie Geist and Luke Kane studied "BRAIDed learning" respectively, Pratima Nayak continued her research and helped out in the PCR studies.

MSU CF Interns Spring & Summer 2006

Joey O'Connor, Aaron Walls, Matt Lincoln and Pratima Nayak
In 2006 Joey O'Connor joined three undergraduate students, Matt Lincoln, Pratima Nayak, and Aaron Walls, who continued their research projects initiated in 2005.

Two teams of undergraduate students joined the lab in Fall 2005 as a part of an apprenticeship course (LBS-347).

Matt Lincoln and Pratima Nayak
This pair of undergraduates characterized the effects of high or low extracellular Cl- on efflux of HCO3-. They were curious about the effect of these gradients of Cl- on the flux of HCO3- through the CFTR channel.

Aaron Walls and Tristin Holton
Aaron and Tristin studied the effects of high or low extracellular Cl- on the movement of Cl- through the CFTR channel (ie. whether high extracellular chloride has a blocker-like effect).

Jeff Quinn and Stacy Drob
Stacy and Jeff tested and developed various protocols associated with extracting genomic DNA from plant tissue and running Arbitrarily Primed PCR to create a DNA fingerprint.

Two research teams of undergraduate students joined the lab in Fall 2004 as a part of an apprenticeship course (LBS-347). They learned techniques of cell culture, ion flux, and microphysiometry as well as became knowledgable about the literature in the field of CF research. They pursued independent research projects described below.

Adam Jablonowski, Katie Sowle and Jamey Hardesty
This group of undergraduates characterized the effects of phoshodiesterase inhibitors, PDEi's, (like Viagra (TM) and papaverine) in CFTR expressing Calu-3 lung submuscosal gland cell lines. They were curious about the effect of these compounds on the conductance of Cl- and HCO3- through the CFTR channel. They collaborated with Joe Maleszewski, a fourth year medical student on this project.

James Howard, MaryBeth Riblett, Luke Kane
This group of undergraduates studied the treatment of externally applied UTP on in NIH-3T3 mouse fibroblast cell lines and the its effect on Cl- and HCO3- ion gradients. They sought to test whether UTP could activate another Cl- channel to carry the Cl- and HCO3- that is no longer being carried by mutant CFTR (ie. activate an alternative pathway for ion flux).

Two research teams of undergraduate students joined the lab in Fall 2003 as a part of an apprenticeship course (LBS-347). They learned techniques of cell culture, ion flux, and microphysiometry as well as became knowledgable about the literature in the field of CF research and pursued independent research projects characterizing CFTR's effect on extracellular pH and antibacterial properties of airway cells.

Andy McCoy, Katie Kruse, and Dan Gutteridge
This group of undergraduates characterized the native antibacterial proteins and compounds (like lactoferrin, lysozyme, etc) secreted by CFTR expressing Calu-3 lung submuscosal gland cell lines and the effect of these compounds on the growth rate of strains of bacteria like E. coli. They also asked what conditions altered the effectiveness of these antibacterials and how activation of CFTR altered their abundance or function.

Angela Wright, John Lambrix, Andy Luea and Erin Fedak
This group of undergraduates studied CFTR expression in C127 mouse mammary epithelia lines and the it's function under different Cl- and HCO3- ion gradients to test whether it serves only as a Cl- channel or if it sometimes functions as an Anion Exchanger carrying exchanging Cl- and HCO3-. They also pursued DNA sequence analysis to determine if any common homologies exist between Anion Exchanger amino acid sequences and motifs and those of CFTR.

2002

Chi Lim (2002-2004, then went to medical school at Wayne State University)

Stephen "Cole" Cahill (2002) The went to medial school in the College of Osteopathic Medicine at Michigan State University.

 
Marija Krha (Massey) (2001-2004 completed M.S. in Physiology and got a job in industry, at Chemetall U.S. Inc. in Ann Arbor, Michigan)

CFTR HCO3-/Cl-  -Research Project
Marija Krha is a graduate student in the CF laboratory who has taken a central role in our research into bicarbonate transport function of CFTR. She is closely examining the capacity for CFTR to carry bicarbonate and chloride. CFTR is a well-characterized Cl- channel and has been found to also carry bicarbonate ions (thus may affect pH). In fact in 2001, new findings by Schmuel Muallem’s laboratory (Choi et al, Nature 2001) indicated bicarbonate transport may be more relevant to the disease cystic fibrosis than even Cl- transport and that the CFTR may in fact be a HCO3-/Cl- exchanger. This functionality linked to HCO3- conductance may prove critical to understanding CF lung disease, the lethal component of cystic fibrosis. Our laboratory is applying our microphysiometry pH technology and Marija is quite focused on participating in this hot new subfield in CF research. As mentioned above, in 2001 we have authored a review of these finding in that will appear in Current Genomics during the summer of 2003.  

Online Citations (Papers and Abstracts)
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Vishal Malhotra (2001-2004, went to medical school at Wayne State University)

Vishal Malhotra a sophomore in the Howard Hughes Undergraduate Scholars Program, joined our research laboratory to learn various recombinant molecular methodologies, and assist in our advanced molecular biology course (LBS-347). Initially he worked on a project to create CFTR-downregulated Calu-3 (Calu-3/LO) and CFTR-knockout Calu-3 (Calu-3/KO) cell lines using both antisense oligonucleotide and antisense vector insertion techniques. Yet recently he's worked on both a Diabetes project studying insulin secretion and a chemical chaperone project testing drugs that can help the expression level and trafficking of mutant 508 CFTR.    

Online Citations (Papers and Abstracts)
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Bob Flood, Haley Jo Jenema, Keith Eaton, Paul Fornetti, Brad Kozel
LBS Interns Fall 2001 (2001-2002)

Five undergraduates joined the lab in Fall 2001 as a part of an apprenticeship course (LBS-347). They learned techniques of molecular biology, cell culture and microphysiometry and pursued independent research projects characterizing CFTR's effect on extracellular pH. This group of undergraduates characterized the native proteins of C127 mouse mammary epithelia lines and the effect of glycerol, a chemical chaperone, on CFTR function.

2000

Aashish A. Shah (2000-2002) Went to medical school at Wayne State University.

In the fall of 2000 Aashish A. Shah, a sophomore in the Johnson Scholars Program and Howard Hughes Undergraduate Scholars Program, joined our research laboratory to learn various recombinant DNA methodologies, and assist in some of our new lines of investigation into Cystic Fibrosis. In addition to working in the research laboratory, Aashish worked in the teaching laboratory of an advanced molecular biology course (LBS-347) and assisted in curricular development of new recombinant DNA inquiries. Aashish continued to develop cell culture skills he initiated with Dr. Karl Olson and learned how to use the Microphysiometer and how to run recombinant molecular biology-related cell experiments. His research in our laboratory suggested that the cystic fibrosis transmembrane conductance regulator (CFTR) may be involved in pH regulation and in the disease state, pH regulation is disrupted resulting in the acidification of the extracellular environment.
 

Online Citations (Papers and Abstracts)
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1999

Chris Singh (Went to medical school at St. Louis University, now works at Vitreoretinal Associates in Seattle Washington, comments from summer of 2000)

"My name is Christopher Singh, and I am currently a second year medical student at Saint Louis University. I got my bachelor of science in human biology at Michigan State University. While there, I had the opportunity to study under and work for Dr. Luckie. His molecular biology class was well taught, and helped to fuel my interest in science. I later worked for him as a teaching assistant, and this helped to strengthen my knowledge of molecular biology, as well as develop my public speaking skills. I also worked on a paper with him concerning CFTR's influence on acid/base regulation in NIH/3T3 fibroblasts and C127 epithelia. This experience sparked an interest in research, and I am currently doing a research project with the ophthalmology department at Saint Louis University."

Representative Publication (Paper):

D.B. Luckie, C.N. Singh, J.J. Wine and J.H. Wilterding (2000) 'CFTR Activation Raises Extracellular pH of NIH/3T3 Mouse Fibroblasts and C127 Epithelial Cells'; submitted to: American Journal of Physiology (Cell Physiology).

Online Citations (Papers and Abstracts)
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Sarah Loznak (now a physician and Clinical Assistant Professor in the Department of Pedeatrics And Human Development at Michigan State University, comments from summer of 2000)

"It is not always the information in textbooks that drives learning. Working in Dr. Luckie's lab, I have found that it is more important what we do with the information and how we use the opportunites we are given to find success in education. Working with him has given me the chance to learn so much more. I was given the opportunity to research how students develop their logical and critical thinking skills. I have developed tests covering such topics as organic chemistry and DNA which serve as a basis for data collection. With this information I have also gained experience in presenting my research at a forum for undergraduate researchers where I was awarded first place honors. As I enter my senior with outstanding grades, time management skills and a wealth of information I know that my experience in working with Dr. Luckie in his lab may be the most important aspect of my educational career."

Representative Publication (Abstract): In 2000 Sarah had 1 abstract published and 1 paper in preparation

S.D. Loznak, D.B. Luckie, J.M. Batzli, D. Ebert-May (2000) Models: Methods Of Describing, Evaluating, And Learning Science. (2000 MSU UURCAF Meeting Abstract, Awarded 1st Place).

1998

John Wilterding (Graduate Student 1998- 2000, now the Chair of the Natural and Physical Sciences Department, and Associate Professor of Biology and Chemistry at Olivet College)

John Wilterding worked in the CF lab for several years and completed his Ph.D. in June of 2000. He was a major contributor to multiple research projects using microphysiometry to study cystic fibrosis as well as innovations in science teaching. He is a co-author on many of the laboratories publications since 1998. John is such a good scientist that he was immediately hired as an Assistant Professor at Olivet College (Olivet, Michigan). In 2001 John sent one of his brillant students, Marija Krha, to join the MSU CF lab to do her graduate work on cystic fibrosis (see below).

Online Citations (Papers and Abstracts)
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Errett Hobbs (1998- 2001, after graduate school at Harvard University, and a postdoc at NIH, he's now working at Lawrence Livermore National Labs in Berkeley California, comments from summer of 2000)

"My name is Errett Hobbs. I am an Honors College junior pursuing degrees in biochemistry/biotechnology and physiology at Michigan State University. I have worked in the Cystic Fibrosis Research Lab in the Department of Physiology for 2/3's of my college career. Our lab's research efforts focus on the use of an instrument called a microphysiometer. This machine, named the Cytosensor by its manufacturer, Molecular Devices, is capable of measuring very small changes in extracellular pH (~0.005 pH unit). We measure the cells' response to the drugs by looking at how their acidification rates change. This coming year I will be graduating with a degree in biochemistry/biotechnology. As a consequence of my work with Dr. Luckie, I am very well prepared to embark on a career in basic research. He has taught me how to operate a silicon chip based device capable of measuring real-time changes in acid efflux, a microphysiometer. I am proficient in basic cell culture techniques, molecular biology, and various biochemical methods such as protein kinetics assays. This year, Iíll be applying techniques of physical chemistry and molecular biology to study acid and chloride efflux in CFTR-knockout cell lines that I will create."

"I have already been listed twice as a co-author on several abstracts and posters. In addition, I have presented a poster at each of the previous two years' Michigan State University Undergraduate Research and Creative Activities Forums. This year I even won an honorable mention. I have also taken an active role in planning the past two years' forums. I have also acquired research support for my junior and senior years in the form of a Howard Hughes Undergraduate Research Scholarship."

Representative Publication (Abstract): In 2000, Errett had 5 abstracts published and 2 papers in preparation

S.R. Hootman, E.C. Hobbs, and D.B. Luckie (1999) Direct measurement of proton efflux from isolated guinea pig pancreatic ducts. Abstract for 1999 American Pancreatic Association Meeting. Pancreas , 19: 424.

Online Citations (Papers and Abstracts)
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Joe Maleszewski
(1998- 2003, after a few years as a Pathology Resident at Johns Hopkins University, he took a cardiovascular pathology position at the Mayo Clinic in Rochester Minnesota, comments from summer of 2000 )

"My name is Joseph J. Maleszewski and I am a senior in the Lyman Briggs School at Michigan State University. I am pursuing a Bachelor of Science degree in physiology as a member of the Honors College. I am writing this letter in hopes of giving you a better idea of the impact that Dr. Douglas Luckie has on his students. All too often, prospective science majors are turned off from their science-track due to the volume and complexity of information they are expected to retain in introductory science courses. This letter is being written, drawing not only from my own experiences with Dr. Luckie, but also from the experiences that other students have had with him. As a teaching assistant under his direction, I have a unique opportunity to listen to students talk about his teaching as well as his mentoring ability. Not only does Dr. Luckie get high praise in the area of teaching, but is also known throughout the Lyman Briggs school as a very 'down to earth' professor that one can easily talk with."

"My own experiences with Dr. Luckie are numerous. I have served as a laboratory assistant under his direction in the Department of Physiology's Cystic Fibrosis Research Lab for three years. In this laboratory, I have been given the opportunity to publish quite frequently, compose research posters, as well as speak at colloquia about our research. As an undergraduate student, it is very rare to get to experience scientific research, in such depth, first hand. Dr. Luckie is very good at being able to provide guidance while still promoting a sense of independence. He is extremely open-minded and listens with a careful ear to all ideas. I have gained a much deeper appreciation for biochemistry and physiology because of my research experience with Dr. Luckie. He has a tremendous ability to relate scientific facts and seemingly useless information to one's everyday life. Previously mentioned, I have served as a teaching assistant for Dr. Luckie for two years. This opportunity has recently given me the chance to publish information on an experimental course, which we have been designing, in educational journals."

"It is very clear to see that my undergraduate experience at MSU would not be the same had Dr. Luckie not been an integral part of my education. I cannot count the times that I have heard students telling other students to 'take Dr. Luckie's course,' because 'it was the only class that I actually feel I learned in.' On a personal level, he has given me opportunities that a majority of undergrads do not receive, and for that I will always be grateful. Dr. Luckie is a unique professor that leaves a very lasting impression on all of his students."

Representative Publication (Abstract): In 2000 Joe had 3 abstracts published and 2 papers in preparation

D.B. Luckie, Maleszewski J.J., Hobbs E.C., Wilterding J.H., Olson L.K. (1999) Extracellular Acidification parallels insulin secretion in pancreatic beta cell lines (INS-1 and HIT). Abstract for 1999 North American Cystic Fibrosis Meeting Abstract. Pediatric Pulmonology Sept. Suppl. 19: 255A.

1997

Indra Neil Sarkar (got a M.S. & Ph.D. at Columbia University, was a Bioinformatics Associate at the American Museum of Natural History in New York, now is Director of Biomedical Informatics at University of Vermont, comments from summer of 2000)

"In 1999 I graduated with a B.Sc. in Microbiology from the Lyman Briggs School at Michigan State University. While as an undergraduate, I had worked in Dr. Luckie's laboratory focusing on computational biology questions - with most of the work focusing on genetic sequence analysis. It was through the work that I was doing in his laboratory that helped spark my interest into bioinformatics and medical informatics. Dr. Luckie provided exceptional guidance to assist my learning of the skills necessary to proceed onto graduate education. Since graduating, I have started graduate education in Medical Informatics at Columbia University's College of Physicians and Surgeons. Some of my current research is focused in the area of genomics. Thanks to Dr. Luckie, I was able to attain the biochemistry skills as an undergraduate which have helped me excel in graduate education."

Representative Publication (Abstract): In 2000, Neil had 1 abstract published and 1 paper in preparation

I.N. Sarkar and D.B. Luckie (1998) Biocomputing: learning how to write programs to solve biological problems. (1998 College of Natural Science Undergraduate Research Forum at Michigan State University; Abstract).

David Chapman (Got PhD at the University of Utah, currently is Medical Science Liaison for Medical Affairs at UCB Pharma, Inc. in St. Louis Missouri, comments from summer of 2000)

"My name is David Chapman, and I am a second year graduate student in the department of pharmacology and toxicology at the University of Utah. My research interests focus on studying interactions between the glutamate and dopamine systems in the brain. This work hopes to extend the existing knowledge about the basal ganglia, and elucidate novel mechanisms for therapeutic intervention in basal ganglia dysfunction, such as Parkinson's Disease and psychostimulant abuse. Research prior to my thesis has given me opportunity to present findings at two national meetings (Experimental Biology and the Society for Neuroscience Annual Meeting), with another abstract submitted for this year's Neuroscience Meeting, and produced a manuscript which has been submitted for publication in the journal of Pharmacology and Experimental Therapeutics. Based on these credentials, I've been awarded a predoctoral fellowship from the American Foundation for Pharmaceutical Education."

"The first of my undergraduate research experiences was in the laboratory of Dr. Doug Luckie. In addition to an introduction to biochemical techniques using cell culture and a microphysiometer, Dr. Luckie helped shape my ability to test and form hypotheses and evaluate scientific work. The overall enthusiasm he has for the scientific process is evident in both his research and teaching opportunities. Briggs, is pretty special. Students get a quality education, in a small liberal arts college-type setting (small classes, professors they can actually interact with, etc), while having all the advantages of the high-power research and scientific toys that are at MSU. You're in a favorable position to cultivate students interests in biochemistry by interacting with them in a fairly intimate setting early in their scientific careers, and then preparing them for the next step through research. I can't think of a much better package."
 
 

Online Citations (Papers and Abstracts)
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