Student Research Projects from Spring 2011
In Spring 2011, 19 student research teams were challenged to propose and pursue a single focused research project for the semester. All students picked a genetic/gene-linked disease and designed PCR-based
diagostic assays for mutations known to cause that disease. Each group wrote manuscripts gave numerous talks and in the end presented their findings at the 6th Annual Briggs Research Symposium as either posters or talks.
Student websites reporting their findings at the end of the independent investigations from Spring Semester 2011
Von Hippel-Lindau (VHL): PCR Based Identification of the R167W Mutation on the VHL Gene for Diagnosis of VHL Disease in IB3 Epithelial Cells.
Muscular Dystrophy: Successful Diagnosis of Duchenne Muscular Dystrophy Using PCR to Detect the Deleted 45th Exon of the Human DMD Gene
Achondroplasia: Using PCR to Successfully Identify the G1138A Point Mutation on the FGFR3 Gene in Achondroplasia Human Cells
GMOs: Identification of GM Zea mays by Detection of Bacillus thuringiensis Cry1A Gene and CaMV 35S Promoter Using PCR
Hemochromatosis: Identification of C282Y Mutation on HFE gene of CALU-3 DNA by Allele Specific PCR and Gel Electrophoresis
Huntington's: Huntington's disease detected in Homo sapien Calu-3 lung epithelial cells based on CAG microsatellite in HTT via PCR
Cystic Fibrosis: Amplification of Cystic Fibrosis W1282X Mutation in HeLa cells using Yaku Primer Design yields Ambiguous Results
Haemochromatosis: Detection of the C282Y Mutation on the HFE Gene Using Allele Specific Primers on Wild Type and Mutated Human DNA
Sickle Cell Anemia: Detection of 1510bp Band Using PCR, Gel Electrophoresis to Diagnose E6V Sickle Cell Anemia in Human CALU-3, BM Cell DNA
Huntington's: PCR of (CAG) repeats on HTT gene in S9 epithelial cells using a threshold HOXD8/10 ladder
Fragile X: PCR Detection of the CGG Trinucleotide Repeats on the FMR1 Gene Using Various Homo Sapien Cells
Huntington's: Assessing the Risk Factor of a Human Cell for HD by Using PCR to Identify the Number of CAG Expansions
Polycystic kidney disease: Identifying F2853S mutation of PKD in human S9 cells via site-specific primers in PCR and Gel Electrophoresis
Alzheimer's: Diagnosing the PS1-E280A mutation in human brain cell of Alzheimer’s patients using PCR and electrophoresis
Bloom Syndrome: Further Findings on BLMash Gene Mutation Identification By Use of PCR and Gel Electrophoresis in B-Lymphocyte Cells
Cerebral Adrenoleukodystrophy: Identification of ΔG529 Mutation in the ALD Protein of S9 Epithelial Cells Using PCR Allele Specific Amplification
