Student Research Projects from Fall 2013
In Fall 2013, ten student research teams were challenged to propose and pursue a single focused research project for the semester. All students studied cystic fibrosis and designed PCR-based
diagostic assays for mutations known to cause that disease. Each group wrote manuscripts gave numerous talks. The reseach assignment involved an extra "control" experiment, where each group was required to also find an existing
published PCR assay for the same gene and replicate the experiment using the published primer sequences. These are often referred to as the "control" primers in the final reports/manuscripts.
NOTE Two student
**groups were also approved to perform sociological experiments where they lived the life of someone with the disease and created short documentary films, only 5-minutes in length, using still photos to communicate the findings
of their "30 Days" sociological experiment
(see links to YouTube, often at the bottom of each group's website).
Student websites reporting their findings at the end of the independent investigations from Fall Semester 2013
R117H: Building an assay for the diagnosis of the R117H mutation using allele specific PCR in IB3 cystic fibrosis cells.
DF508: Improving Primer Design in Allele Specific PCR Diagnostic Assays Seeking Deletion Mutations Using CFTR ΔF508 Locus.
G551D: Genotypic Detection of G551D Mutation for Cystic Fibrosis in Human IB3-1 Cells Using Yaku Designed PCR Primers
**W1282X: Identification of W1282X mutation of CF in Human IB3 Epithelial Cells Using Allele Specific PCR and Yaku Primers
3849: Diagnosing the 3849+10kb C>T Mutation of the CFTR Gene in Human IB3 cells using ASPCR and Electrophoresis.
**Canine CFTR mutations: PCR and Gel Electrophoretic Analysis of Canine Cells to Reveal R1456W and R812W Missense Mutations on CFTR Gene
