Student Research Projects from Fall 2009 (this semester's themes: Paris and cystic fibrosis 2.0).
In Fall 2009, research teams of four students each were challenged to propose and pursue a single focused research project for the semester. All students read & presented scientific publications in a weekly journal club on cystic fibrosis and PCR.
One group, Creative Crush, pursued a special "Paris Option" where half of their research would occur at MSU and the second half in Paris on Study Abroad.
The rest of the students in the course, some 11 groups, focused using two strategies [Yaku vs Traditional] in creating PCR-based diangostic assays for 11 mutations known to cause cystic fibrosis.
The work of these groups in Fall was a continuation and completion of some of the work students began in Spring 2009. Student teams pursued a 14-week investigation and
presented their findings in various forms (web sites, interviews, talks and multiple drafts of a scientific manuscript along the way) as well as at the 4th Annual Briggs Research Symposium as either posters or talks.
Student Powerpoint slides reporting their proposal for Art & Science investigations from Fall Semester 2009
ANS genes in Paintings: The detection of Anthocyanidin synthase in Rosa hybrida cultivar and Paris Art using PCR
Student websites reporting their findings at the end of the CF & PCR Stream independent investigations from Fall Semester 2009
G542X-CFTR Traditional: Traditional Primer Method Confirms the Absence of the G542X Mutation in IB3 Cells Using PCR and Gel Electrophoresis
G542X-CFTR Yaku: Yaku Primer Design Method for PCR Inconclusive in Identification of the G542X Mutation of CFTR in IB3 Cells
A455E-CFTR Traditional: An Assay Demonstrating a 591 bp Band for the wt CFTR Sequence at the A455E Locus in CF Patients using S9 Cells and PCR
A455E-CFTR Yaku: Gene Expression of band amplification in S9 cell line of CF patient for A455E mutation employing Allele Specific PCR
1078delT-CFTR: Identification of Mutation 1078delT in the CFTR Gene in S9 epithelial cell from a CF patient Using Allele Specific PCR
1078delT-CFTR: Genotypic Identification of CFTR 1078delT Mutation in IB3 cells of Cystic Fibrosis Patients Using PCR Replication
W1282X-CFTR Traditional: Inconclusive Detection of CFTR Mutation W1282X in Infected Human IB3 Cells Using PCR and Gel Electrophoresis
W1282X-CFTR Yaku: Detection of CFTR mutation W1282X in IB3 human DNA with a band length of 884 base pairs using PCR and Gel Electrophoresis.
E60X-CFTR Traditional: Development of Diagnostic Assay to Test for Absence of E60X Mutation in Wild Type IB3 Cells Using PCR Amplification
E60X-CFTR Yaku: Development of Assay for E60X CFTR Mutation in IB3 Cell Lines Using PCR and Statistical Analysis of Medical Compliance
G85E-CFTR both Yaku and Traditional: Greater Specificity in Yaku versus Traditional Primer Design in AS-PCR Diagnostic of G85E CFTR mutation in IB3 Cells
